Kfl00048_0230 (kfl00048_0230_v1.1)


Aliases : kfl00048_0230_v1.1

Description : (at1g03310 : 522.0) Encodes a protein with strong similarity to isoamylase (EC:3.2.1.68) however lacks critical residues known to be important for activity. Appears to co localize with ISA1 in the chloroplast isoamylase complex. Mutations in this gene cause the loss of detectable isoamylase activity and the disruption of normal starch structure. It has been postulated that AtISA2 interacts with AtISA1 to form the Iso1 complex.; debranching enzyme 1 (DBE1); FUNCTIONS IN: isoamylase activity, alpha-amylase activity; INVOLVED IN: carbohydrate metabolic process, amylopectin biosynthetic process; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 13, N-terminal (InterPro:IPR004193), Immunoglobulin E-set (InterPro:IPR014756), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047); BEST Arabidopsis thaliana protein match is: isoamylase 3 (TAIR:AT4G09020.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). & (reliability: 1044.0) & (original description: no original description)


Gene families : OG_42_0001338 (Orthogroups_2024-Update) Phylogenetic Tree(s): OG0001338_tree

Sequence : coding (download), protein (download)


Note:Only the main profile, including all conditions, is shown. Additional statistics and tissue specific profiles are available here.


Type Description Actions
Neighborhood Klebsormidium release: Kfl00048_0230
Cluster HCCA clusters: Cluster_82

Target Alias Description ECC score Gene Family Method Actions
At4g09020 No alias Isoamylase 3, chloroplastic... 0.02 Orthogroups_2024-Update
GRMZM2G150796 No alias isoamylase 3 0.02 Orthogroups_2024-Update
Glyma.04G098900 No alias isoamylase 3 0.02 Orthogroups_2024-Update
Glyma.08G028400 No alias isoamylase 1 0.03 Orthogroups_2024-Update
Kfl00439_0040 kfl00439_0040_v1.1 (at2g39930 : 925.0) Encodes an isoamylase-type... 0.02 Orthogroups_2024-Update
LOC_Os05g32710 No alias Alpha amylase, catalytic domain containing protein, expressed 0.02 Orthogroups_2024-Update
Potri.002G215400 No alias debranching enzyme 1 0.02 Orthogroups_2024-Update
Potri.002G219900 No alias debranching enzyme 1 0.02 Orthogroups_2024-Update
Seita.6G211200.1 No alias component *(ISA1) of ISA1-ISA2 isoamylase heterodimer 0.02 Orthogroups_2024-Update
Sobic.009G127500.2 No alias component *(ISA2) of ISA1-ISA2 isoamylase heterodimer 0.03 Orthogroups_2024-Update
evm.model.tig00000955.11 No alias (at4g09020 : 389.0) Encodes an isoamylase-like protein.... 0.02 Orthogroups_2024-Update

Type GO Term Name Evidence Source
MF GO:0003824 catalytic activity IEA InterProScan predictions
MF GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds IEA InterProScan predictions
BP GO:0005975 carbohydrate metabolic process IEA InterProScan predictions
Type GO Term Name Evidence Source
MF GO:0000166 nucleotide binding IEP Predicted GO
MF GO:0002161 aminoacyl-tRNA editing activity IEP Predicted GO
MF GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity IEP Predicted GO
MF GO:0004146 dihydrofolate reductase activity IEP Predicted GO
MF GO:0004177 aminopeptidase activity IEP Predicted GO
MF GO:0004427 inorganic diphosphatase activity IEP Predicted GO
MF GO:0004470 malic enzyme activity IEP Predicted GO
MF GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity IEP Predicted GO
MF GO:0004576 oligosaccharyl transferase activity IEP Predicted GO
MF GO:0004645 phosphorylase activity IEP Predicted GO
MF GO:0004812 aminoacyl-tRNA ligase activity IEP Predicted GO
MF GO:0004820 glycine-tRNA ligase activity IEP Predicted GO
MF GO:0005524 ATP binding IEP Predicted GO
CC GO:0005737 cytoplasm IEP Predicted GO
BP GO:0006082 organic acid metabolic process IEP Predicted GO
BP GO:0006139 nucleobase-containing compound metabolic process IEP Predicted GO
BP GO:0006399 tRNA metabolic process IEP Predicted GO
BP GO:0006418 tRNA aminoacylation for protein translation IEP Predicted GO
BP GO:0006426 glycyl-tRNA aminoacylation IEP Predicted GO
BP GO:0006486 protein glycosylation IEP Predicted GO
BP GO:0006520 cellular amino acid metabolic process IEP Predicted GO
BP GO:0006528 asparagine metabolic process IEP Predicted GO
BP GO:0006529 asparagine biosynthetic process IEP Predicted GO
BP GO:0006725 cellular aromatic compound metabolic process IEP Predicted GO
BP GO:0006732 coenzyme metabolic process IEP Predicted GO
BP GO:0006760 folic acid-containing compound metabolic process IEP Predicted GO
BP GO:0006801 superoxide metabolic process IEP Predicted GO
BP GO:0006807 nitrogen compound metabolic process IEP Predicted GO
BP GO:0006891 intra-Golgi vesicle-mediated transport IEP Predicted GO
MF GO:0008144 drug binding IEP Predicted GO
MF GO:0008184 glycogen phosphorylase activity IEP Predicted GO
MF GO:0008236 serine-type peptidase activity IEP Predicted GO
CC GO:0008250 oligosaccharyltransferase complex IEP Predicted GO
BP GO:0009396 folic acid-containing compound biosynthetic process IEP Predicted GO
MF GO:0009678 hydrogen-translocating pyrophosphatase activity IEP Predicted GO
MF GO:0015098 molybdate ion transmembrane transporter activity IEP Predicted GO
MF GO:0015399 primary active transmembrane transporter activity IEP Predicted GO
MF GO:0015405 P-P-bond-hydrolysis-driven transmembrane transporter activity IEP Predicted GO
BP GO:0015689 molybdate ion transport IEP Predicted GO
BP GO:0016070 RNA metabolic process IEP Predicted GO
MF GO:0016491 oxidoreductase activity IEP Predicted GO
MF GO:0016614 oxidoreductase activity, acting on CH-OH group of donors IEP Predicted GO
MF GO:0016615 malate dehydrogenase activity IEP Predicted GO
MF GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor IEP Predicted GO
MF GO:0016645 oxidoreductase activity, acting on the CH-NH group of donors IEP Predicted GO
MF GO:0016646 oxidoreductase activity, acting on the CH-NH group of donors, NAD or NADP as acceptor IEP Predicted GO
MF GO:0016758 transferase activity, transferring hexosyl groups IEP Predicted GO
MF GO:0016772 transferase activity, transferring phosphorus-containing groups IEP Predicted GO
MF GO:0016779 nucleotidyltransferase activity IEP Predicted GO
MF GO:0016874 ligase activity IEP Predicted GO
MF GO:0016875 ligase activity, forming carbon-oxygen bonds IEP Predicted GO
MF GO:0016884 carbon-nitrogen ligase activity, with glutamine as amido-N-donor IEP Predicted GO
MF GO:0017076 purine nucleotide binding IEP Predicted GO
CC GO:0017119 Golgi transport complex IEP Predicted GO
MF GO:0017171 serine hydrolase activity IEP Predicted GO
BP GO:0019321 pentose metabolic process IEP Predicted GO
BP GO:0019566 arabinose metabolic process IEP Predicted GO
BP GO:0019752 carboxylic acid metabolic process IEP Predicted GO
CC GO:0019867 outer membrane IEP Predicted GO
MF GO:0030170 pyridoxal phosphate binding IEP Predicted GO
MF GO:0030554 adenyl nucleotide binding IEP Predicted GO
BP GO:0032324 molybdopterin cofactor biosynthetic process IEP Predicted GO
MF GO:0032553 ribonucleotide binding IEP Predicted GO
MF GO:0032555 purine ribonucleotide binding IEP Predicted GO
MF GO:0032559 adenyl ribonucleotide binding IEP Predicted GO
BP GO:0034641 cellular nitrogen compound metabolic process IEP Predicted GO
BP GO:0034660 ncRNA metabolic process IEP Predicted GO
CC GO:0034998 oligosaccharyltransferase I complex IEP Predicted GO
MF GO:0035639 purine ribonucleoside triphosphate binding IEP Predicted GO
MF GO:0036094 small molecule binding IEP Predicted GO
BP GO:0042278 purine nucleoside metabolic process IEP Predicted GO
BP GO:0042558 pteridine-containing compound metabolic process IEP Predicted GO
BP GO:0042559 pteridine-containing compound biosynthetic process IEP Predicted GO
BP GO:0043038 amino acid activation IEP Predicted GO
BP GO:0043039 tRNA aminoacylation IEP Predicted GO
MF GO:0043167 ion binding IEP Predicted GO
MF GO:0043168 anion binding IEP Predicted GO
BP GO:0043170 macromolecule metabolic process IEP Predicted GO
BP GO:0043413 macromolecule glycosylation IEP Predicted GO
BP GO:0043436 oxoacid metabolic process IEP Predicted GO
BP GO:0043545 molybdopterin cofactor metabolic process IEP Predicted GO
BP GO:0044237 cellular metabolic process IEP Predicted GO
BP GO:0044281 small molecule metabolic process IEP Predicted GO
BP GO:0046039 GTP metabolic process IEP Predicted GO
BP GO:0046128 purine ribonucleoside metabolic process IEP Predicted GO
BP GO:0046373 L-arabinose metabolic process IEP Predicted GO
BP GO:0046483 heterocycle metabolic process IEP Predicted GO
MF GO:0046556 alpha-L-arabinofuranosidase activity IEP Predicted GO
BP GO:0046653 tetrahydrofolate metabolic process IEP Predicted GO
BP GO:0046654 tetrahydrofolate biosynthetic process IEP Predicted GO
BP GO:0051189 prosthetic group metabolic process IEP Predicted GO
BP GO:0070085 glycosylation IEP Predicted GO
MF GO:0070279 vitamin B6 binding IEP Predicted GO
MF GO:0070569 uridylyltransferase activity IEP Predicted GO
BP GO:0090304 nucleic acid metabolic process IEP Predicted GO
MF GO:0097159 organic cyclic compound binding IEP Predicted GO
MF GO:0097367 carbohydrate derivative binding IEP Predicted GO
MF GO:0140098 catalytic activity, acting on RNA IEP Predicted GO
MF GO:0140101 catalytic activity, acting on a tRNA IEP Predicted GO
BP GO:1901068 guanosine-containing compound metabolic process IEP Predicted GO
MF GO:1901265 nucleoside phosphate binding IEP Predicted GO
BP GO:1901360 organic cyclic compound metabolic process IEP Predicted GO
MF GO:1901363 heterocyclic compound binding IEP Predicted GO
BP GO:1901564 organonitrogen compound metabolic process IEP Predicted GO
InterPro domains Description Start Stop
IPR004193 Glyco_hydro_13_N 172 264
IPR006047 Glyco_hydro_13_cat_dom 332 439
No external refs found!