- Home
- Species
- Pseudotsuga menziesii
- Sequence
- PSME_00029548-RA
PSME_00029548-RA
Description : (at4g09020 : 409.0) Encodes an isoamylase-like protein. Mutant studies show that the gene is strongly involved in starch breakdown. A GUS-protein fusion product was shown to localize to the surface of chloroplastic structures reminiscent of starch granules. In the mutants, the chloroplastic α-amylase AMY3 is upregulated.; isoamylase 3 (ISA3); FUNCTIONS IN: isoamylase activity, alpha-amylase activity; INVOLVED IN: carbohydrate metabolic process, starch catabolic process; LOCATED IN: chloroplast, chloroplast stroma, chloroplast starch grain; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Glycosyl hydrolase, family 13, all-beta (InterPro:IPR013780), Immunoglobulin-like fold (InterPro:IPR013783), Glycoside hydrolase, family 13, N-terminal (InterPro:IPR004193), Immunoglobulin E-set (InterPro:IPR014756), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781), Glycosyl hydrolase, family 13, catalytic domain (InterPro:IPR006047); BEST Arabidopsis thaliana protein match is: isoamylase 1 (TAIR:AT2G39930.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). & (reliability: 818.0) & (original description: no original description)
Expression Profile
Note:Only the main profile, including all conditions, is shown. Additional statistics and tissue specific profiles are available here.
Co-expression Networks
| Type | Description | Actions |
| Neighborhood | Pseudotsuga release: PSME_00029548-RA | |
| Cluster | HCCA clusters: Cluster_162 | |
Expression Context Conservation (ECC)
| Target | Alias | Description | ECC score | Gene Family Method | Actions |
| Glyma.03G151200 | No alias | debranching enzyme 1 | 0.03 | Orthogroups_2024-Update | |
| Potri.018G132500 | No alias | isoamylase 1 | 0.03 | Orthogroups_2024-Update | |
| Seita.2G237100.1 | No alias | starch-debranching isoamylase *(ISA3) | 0.02 | Orthogroups_2024-Update | |
| Sopen06g003990 | No alias | Alpha amylase, catalytic domain | 0.02 | Orthogroups_2024-Update | |
Functional Annotation
| Type | GO Term | Name | Evidence | Source |
| MF | GO:0003674 | molecular_function | None | Extended |
| MF | GO:0003824 | catalytic activity | IEA | InterProScan predictions |
| MF | GO:0004553 | hydrolase activity, hydrolyzing O-glycosyl compounds | IEA | InterProScan predictions |
| BP | GO:0005975 | carbohydrate metabolic process | IEA | InterProScan predictions |
| BP | GO:0008150 | biological_process | None | Extended |
| BP | GO:0008152 | metabolic process | None | Extended |
| MF | GO:0016787 | hydrolase activity | None | Extended |
| MF | GO:0016798 | hydrolase activity, acting on glycosyl bonds | None | Extended |
| BP | GO:0044238 | primary metabolic process | None | Extended |
| BP | GO:0071704 | organic substance metabolic process | None | Extended |
| Type | GO Term | Name | Evidence | Source |
| MF | GO:0003968 | RNA-directed 5'-3' RNA polymerase activity | IEP | Predicted GO |
| MF | GO:0004476 | mannose-6-phosphate isomerase activity | IEP | Predicted GO |
| MF | GO:0004525 | ribonuclease III activity | IEP | Predicted GO |
| MF | GO:0008483 | transaminase activity | IEP | Predicted GO |
| MF | GO:0016769 | transferase activity, transferring nitrogenous groups | IEP | Predicted GO |
| MF | GO:0016861 | intramolecular oxidoreductase activity, interconverting aldoses and ketoses | IEP | Predicted GO |
| MF | GO:0032296 | double-stranded RNA-specific ribonuclease activity | IEP | Predicted GO |
| BP | GO:0055085 | transmembrane transport | IEP | Predicted GO |
| MF | GO:0140098 | catalytic activity, acting on RNA | IEP | Predicted GO |
| InterPro domains | Description | Start | Stop |
| IPR006047 | Glyco_hydro_13_cat_dom | 224 | 344 |
| IPR004193 | Glyco_hydro_13_N | 20 | 119 |
